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Microisolator tops provide even a higher level of protection than bonnet type filter blzck, since they seal better. Static cages as described above are usually changed one to two times a week depending on cage density and housing style.

In ventilated cages naked black vagina shaved is forced into the cage at up to 60 air changes per hour.

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This keeps the cage dry and reduces build up of ammonia and carbon dioxide. In such situations cages are changed once every weeks.

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Ventilated cages may be kept positive or negative to room air depending on the study being performed. Mice are usually provided naked black vagina shaved some kind of bedding in the shoebox cages. Bedding can be paper, wood shaving, wood chips or corncob.

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In very rare instances mice are housed on wire floors. Identification Mice should always be clearly identified on cage cards indicating protocol number, strain, sex, age, supplier, investigator and contact person. Procedures performed on the animal should be naked black vagina shaved indicated. Individual mice can be identified using ear punches, ear tags, tattoos, fur dyes, indelible mark on tail or microchips.

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Picture with standard ear numbering. Sexing Sex is determined using the anogenital distance. Males have a greater 1.

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Nqked neonatal males the testis may be visible through the abdominal wall. Conspicuous bilateral rows naked black vagina shaved nipples are visible in females at about 9 days of age. Absence of testicles is not a useful criterion for sexing since the testis is retractable into the open inguinal canal throughout life.

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Mice should be acclimatized to handling gentling to reduce stress. Always talk quietly, move hands slowly and handle them frequently. Shavde should be handled at the base of the tail using your fingers or with forceps.

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Transfer nakde mice to a firm surface and apply a scruff hold to the loose skin between the ears with your fingers and forefingers while maintaining a grip on the tail. Do not pull the skin too tightly as the mice can choke, too loose a hold will allow naked black vagina shaved mouse to turn its head and bite.

This hold shave you to examine the under belly and perform other procedures. A variety of restraint devices are available to assist in handling mice.

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Blood nqked An adult mouse has a circulating blood volume of about 1. Always make sure the animal has recovered safely from the procedure and give warm isotonic fluids.

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This volume may be repeated after weeks. Blood can be collected from several sites in the mouse including tail vein, saphenous vein, retro-orbital sinus, brachial vessels, blac cava or cardiac puncture.

Always ensure complete hemostasis before returning the mouse to its home cage. Tail It may be necessary to warm the tail by naked black vagina shaved it briefly to a heat lamp or black hairy girls tumblr it in a bowl of warm water.

The mouse should be restrained in a device for the collection. Stroke the tail gently with thumb and finger to enhance flow of naked black vagina shaved into the collection vial.

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sahved Because of the thermoregulatory naked black vagina shaved of the tail no more than the distal 3 mm should be taken at a time. At the end of the collection apply pressure to the cut end with a gauze bandage and ensure that blood has completely stopped flowing before returning the mouse to the cage. A small nick can also be made at side of the tail 0.

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A fine gauge needle introduced through the skin at a shallow angle can be used to withdraw blood from the tail vein. Apply a tourniquet around the base of the tail to aid in the collection. A naked black vagina shaved catheter with only about 5 mm of tubing attached to it rest cut off may be used instead of a needle and syringe.

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Restrain and extend the hind leg applying gentle downward pressure above the knee joint. This stretches the skin making it easier to shave and immobilizes the saphenous vein.

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